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cd223 antibody, anti-human, reafinity  (Miltenyi Biotec)


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    Miltenyi Biotec cd223 antibody, anti-human, reafinity
    Cd223 Antibody, Anti Human, Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd223+antibody/CD223+Antibody%2C+anti-human%2C+REAfinity/custom%40130-135-351%4042526444
    Average 93 stars, based on 96 article reviews
    cd223 antibody, anti-human, reafinity - by Bioz Stars, 2026-09
    93/100 stars

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    Single Cell:

    Article Title: Gut Microbiota Modulation through Akkermansia spp . Supplementation Increases CAR T-cell Potency
    Article Snippet: Briefly, after obtaining single-cell suspensions, tumors were stained for 30 minutes at 4°C with a mix of antibodies containing anti-CAR T-cell CD19 (Cat. No. 130-127-342, clone REA1297, Miltenyi), anti-CCR6 (Cat. No. 566130, clone 11A9, BD), anti-CCR7 (Cat. No. 566437, clone 3D12 RUO, BD), anti-CD11b (Cat. No. 301325, clone ICRF44), anti-CD11c (Cat. No. 130-128-247, clone REA618, Miltenyi), anti-CD123 (Cat. No. 130-115-272, clone REA918, Miltenyi), anti-CD127 (Cat. No. 351363, clone A019D5, BioLegend), anti-CD141 (Cat. No. 344113, clone M80, BioLegend), anti-CD16 (Cat. No. 130-113-395, clone REA423, Miltenyi), anti-CD163 (Cat. No. 333631, clone GHI/61, BioLegend), anti-CD19 (Cat. No. 612916, clone SJ25C1, BD), anti-CD1c (Cat. No. 331537, clone L161, BioLegend), anti-CD20 (Cat. No. 612906, clone 2H7, BD), anti-CD206 (Cat. No. 130-126-623, clone DCN228, Miltenyi), anti-CD25 (Cat. No. 356145, clone M-A251, BioLegend), anti-CD3 (Cat. No. 130-113-142, clone REA613, Miltenyi), anti-CD38 (Cat. No. 303549, clone HIT2, BioLegend), anti-CD39 (Cat. No. 749967, clone TU66, BD), anti-CD4 (Cat. No. 344669, clone SK3, BioLegend), anti-CD45 (Cat. No. 130-113-120, clone 5B1, Miltenyi), CD45RA (Cat. No. 740298, clone HI100, BD), anti-CD56 (Cat. No. 362513, clone 5.1H11, BioLegend), anti-CD66b (Cat. No. 305111, clone G10F5, BioLegend), CD79b (Cat. No. 612814, clone CB3-1, BD), anti-CD8 (Cat. No. 344759, clone SK1, BioLegend), anti-CXCR3 (Cat. No. 353755, clone G025H7, BioLegend), anti-CXCR5 (Cat. No. 356941, clone J252D4, BioLegend), anti-FOLR2 (Cat. No. 391705, clone 94b/FOLR2, BioLegend), anti–HLA-DR (Cat. No. 307683, clone L243, BioLegend), anti–PD-1 (Cat. No. 329927, clone EH12.2H7, BioLegend), anti-TCF1 (Cat. No. 905115, Cell Signaling Technology), anti–TIM-3 (Cat. No. 345027, clone F38-2E2, BioLegend), anti-TREM2 (Cat. No. FAB17291G, clone 237920, RD), and Viability (Cat. No. 423105, BioLegend). .. After obtaining single-cell suspensions, BM mononuclear cells were stained with a mix of antibodies for 30 minutes at 4°C, comprising CD223 antibody, anti-human, REAfinity (Cat. No. 130-118-549, Miltenyi); BUV395 Mouse Anti-Human CD41a (Cat. No. 740295, BD); BUV496 Mouse Anti-Human CD90 (Cat. No. 741160, BD); BUV661 Mouse Anti-Human CD39 (Cat. No. 749967, BD); BUV737 Mouse Anti-Human CD7 (Cat. No. 753774, BD); Brilliant Violet 421 anti-human CD34 Antibody (Cat. No. 343610, BioLegend); Brilliant Violet 570 anti-human CD3 Antibody (Cat. No. 300436, BioLegend); BV605 Mouse Anti-Human CD56 (Cat. No. 562779, BD); Brilliant Violet 650 anti-human CD16 Antibody (Cat. No. 302042, BioLegend); BV786 Mouse Anti-Human CD33 (Cat. No. 740974, BD); PerCP anti-human CD45 Antibody (Cat. No. 368506, BioLegend); PE/Fire 640 anti-human CD19 Antibody (BioLegend); CD19 CAR FMC63 Idiotype Antibody, REAfinity (Cat. No. 130-127-342, Miltenyi); BUV615 Rat Anti-Human CD115 (CSF-1R; Cat. No. 751279, BD); BB515 Armenian Hamster Anti-ICOS (CD278; Cat. No. 565880, BD); BUV805 Mouse Anti-Human CD38 (Cat. No. 742074, BD); and BV510 Mouse Anti-Human CD279 (PD-1; Cat. No. 563076, BD). .. FCS files were exported using FlowJo software.

    Article Title: Gut microbiota modulation through Akkermansia spp. supplementation increases CAR-T cell potency
    Article Snippet: Briefly, after obtaining single cell suspensions, tumors were stained for 30 minutes at 4°C with a mix of antibodies containing: anti-CAR-T CD19 (Cat. No. 130-127-342, clone REA1297, Miltenyi), anti-CCR6 (Cat. No. 566130, clone 11A9, BD), anti-CCR7 (Cat No. 566437, clone 3D12 RUO, BD), anti-CD11b (Cat. No. 301325, clone ICRF44), anti-CD11c (Cat. No. 130-128-247, clone REA618, Miltenyi), anti-CD123 (Cat. No. 130-115-272, clone REA918, Miltenyi), anti-CD127 (Cat. No. 351363, clone A019D5, Biolegend), anti-CD141 (Cat. No. 344113, clone M80, Biolegend), anti-CD16 (Cat. No. 130-113-395, clone REA423, Miltenyi), anti-CD163 (Cat. No. 333631, clone GHI/61, Biolegend), anti-CD19 (Cat. No. 612916, clone SJ25C1, BD), anti-CD1c (Cat. No. 331537, clone L161, Biolegend), anti-CD20 (Cat. No. 612906, clone 2H7, BD), anti-CD206 (Cat. No. 130-126- 623, clone DCN228, Miltenyi), anti-CD25 (Cat. No. 356145, clone M-A251, Biolegend), anti-CD3 (Cat. No. 130-113-142, clone REA613, Miltenyi), anti-CD38 (Cat. No. 303549, clone HIT2, Biolegend), antiCD39 (Cat. No. 749967, clone TU66, BD), anti-CD4 (Cat. No. 344669, clone SK3, Biolegend), antiCD45 (Cat. No. 130-113-120, clone 5B1, Miltenyi), CD45RA (Cat. No. 740298, clone HI100, BD), antiCD56 (Cat. No. 362513, clone 5.1H11, Biolegend), anti-CD66b (Cat. No. 305111, clone G10F5, Biolegend), CD79b (Cat. No. 612814, clone CB3-1, BD), anti-CD8 (Cat. No. 344759, clone SK1, Biolegend), anti-CXCR3 (Cat. No. 353755, clone G025H7, Biolegend), anti-CXCR5 (Cat. No. 356941, clone J252D4, Biolegend), anti-FOLR2 (Cat. No. 391705, clone 94b/FOLR2, Biolegend), anti-HLA-DR (Cat. No. 307683, clone L243, Biolegend), anti-PD-1 (Cat. No. 329927, clone EH12.2H7, Biolegend), anti-TCF1 (Cat. No. 905115, Cell Signaling), anti-TIM-3 (Cat. No. 345027, clone F38-2E2, Biolegend), anti-TREM2 (Cat. No. FAB17291G, clone 237920, RD), Viability (Cat. No. 423105, Biolegend). .. After obtaining single cell suspensions, bone marrow mononuclear cells were stained with a mix of antibodies for 30 minutes at 4°C, comprising: CD223 Antibody, anti-human, REAfinityTM (Cat. No. 130- 118-549, Miltenyi), BUV395 Mouse Anti-Human CD41a (Cat. No. 740295, BD), BUV496 Mouse AntiHuman CD90 (Cat. No. 741160, BD), BUV661 Mouse Anti-Human CD39 (Cat. No. 749967, BD), BUV737 Mouse Anti-Human CD7 (Cat. No. 753774, BD), Brilliant Violet 421TM anti-human CD34 Antibody (Cat. No. 343610, Biolegend), Brilliant Violet 570TM anti-human CD3 Antibody (Cat. No. 300436, Biolegend), BV605 Mouse Anti-Human CD56 (Cat. No. 562779, BD), Brilliant Violet 650TM anti-human CD16 Antibody (302042, Biolegend), BV786 Mouse Anti-Human CD33 (Cat. No. 740974, BD), PerCP anti-human CD45 Antibody (Cat. No. 368506, Biolegend), PE/FireTM 640 anti-human CD19 Antibody (Biolegend), CD19 CAR FMC63 Idiotype Antibody, REAfinityTM (Cat. No. 130-127-342, Miltenyi), BUV615 Rat Anti-Human CD115 (CSF-1R) (Cat. No. 751279, BD), BB515 Armenian Hamster Anti-ICOS (CD278) (Cat. No. 565880, BD), BUV805 Mouse Anti-Human CD38 (Cat. No.742074, BD), BV510 Mouse Anti-Human CD279 (PD-1) (Cat. No. 563076, BD). .. FCS files were exported using FlowJO software.

    Staining:

    Article Title: Gut Microbiota Modulation through Akkermansia spp . Supplementation Increases CAR T-cell Potency
    Article Snippet: Briefly, after obtaining single-cell suspensions, tumors were stained for 30 minutes at 4°C with a mix of antibodies containing anti-CAR T-cell CD19 (Cat. No. 130-127-342, clone REA1297, Miltenyi), anti-CCR6 (Cat. No. 566130, clone 11A9, BD), anti-CCR7 (Cat. No. 566437, clone 3D12 RUO, BD), anti-CD11b (Cat. No. 301325, clone ICRF44), anti-CD11c (Cat. No. 130-128-247, clone REA618, Miltenyi), anti-CD123 (Cat. No. 130-115-272, clone REA918, Miltenyi), anti-CD127 (Cat. No. 351363, clone A019D5, BioLegend), anti-CD141 (Cat. No. 344113, clone M80, BioLegend), anti-CD16 (Cat. No. 130-113-395, clone REA423, Miltenyi), anti-CD163 (Cat. No. 333631, clone GHI/61, BioLegend), anti-CD19 (Cat. No. 612916, clone SJ25C1, BD), anti-CD1c (Cat. No. 331537, clone L161, BioLegend), anti-CD20 (Cat. No. 612906, clone 2H7, BD), anti-CD206 (Cat. No. 130-126-623, clone DCN228, Miltenyi), anti-CD25 (Cat. No. 356145, clone M-A251, BioLegend), anti-CD3 (Cat. No. 130-113-142, clone REA613, Miltenyi), anti-CD38 (Cat. No. 303549, clone HIT2, BioLegend), anti-CD39 (Cat. No. 749967, clone TU66, BD), anti-CD4 (Cat. No. 344669, clone SK3, BioLegend), anti-CD45 (Cat. No. 130-113-120, clone 5B1, Miltenyi), CD45RA (Cat. No. 740298, clone HI100, BD), anti-CD56 (Cat. No. 362513, clone 5.1H11, BioLegend), anti-CD66b (Cat. No. 305111, clone G10F5, BioLegend), CD79b (Cat. No. 612814, clone CB3-1, BD), anti-CD8 (Cat. No. 344759, clone SK1, BioLegend), anti-CXCR3 (Cat. No. 353755, clone G025H7, BioLegend), anti-CXCR5 (Cat. No. 356941, clone J252D4, BioLegend), anti-FOLR2 (Cat. No. 391705, clone 94b/FOLR2, BioLegend), anti–HLA-DR (Cat. No. 307683, clone L243, BioLegend), anti–PD-1 (Cat. No. 329927, clone EH12.2H7, BioLegend), anti-TCF1 (Cat. No. 905115, Cell Signaling Technology), anti–TIM-3 (Cat. No. 345027, clone F38-2E2, BioLegend), anti-TREM2 (Cat. No. FAB17291G, clone 237920, RD), and Viability (Cat. No. 423105, BioLegend). .. After obtaining single-cell suspensions, BM mononuclear cells were stained with a mix of antibodies for 30 minutes at 4°C, comprising CD223 antibody, anti-human, REAfinity (Cat. No. 130-118-549, Miltenyi); BUV395 Mouse Anti-Human CD41a (Cat. No. 740295, BD); BUV496 Mouse Anti-Human CD90 (Cat. No. 741160, BD); BUV661 Mouse Anti-Human CD39 (Cat. No. 749967, BD); BUV737 Mouse Anti-Human CD7 (Cat. No. 753774, BD); Brilliant Violet 421 anti-human CD34 Antibody (Cat. No. 343610, BioLegend); Brilliant Violet 570 anti-human CD3 Antibody (Cat. No. 300436, BioLegend); BV605 Mouse Anti-Human CD56 (Cat. No. 562779, BD); Brilliant Violet 650 anti-human CD16 Antibody (Cat. No. 302042, BioLegend); BV786 Mouse Anti-Human CD33 (Cat. No. 740974, BD); PerCP anti-human CD45 Antibody (Cat. No. 368506, BioLegend); PE/Fire 640 anti-human CD19 Antibody (BioLegend); CD19 CAR FMC63 Idiotype Antibody, REAfinity (Cat. No. 130-127-342, Miltenyi); BUV615 Rat Anti-Human CD115 (CSF-1R; Cat. No. 751279, BD); BB515 Armenian Hamster Anti-ICOS (CD278; Cat. No. 565880, BD); BUV805 Mouse Anti-Human CD38 (Cat. No. 742074, BD); and BV510 Mouse Anti-Human CD279 (PD-1; Cat. No. 563076, BD). .. FCS files were exported using FlowJo software.

    Article Title: Gut microbiota modulation through Akkermansia spp. supplementation increases CAR-T cell potency
    Article Snippet: Briefly, after obtaining single cell suspensions, tumors were stained for 30 minutes at 4°C with a mix of antibodies containing: anti-CAR-T CD19 (Cat. No. 130-127-342, clone REA1297, Miltenyi), anti-CCR6 (Cat. No. 566130, clone 11A9, BD), anti-CCR7 (Cat No. 566437, clone 3D12 RUO, BD), anti-CD11b (Cat. No. 301325, clone ICRF44), anti-CD11c (Cat. No. 130-128-247, clone REA618, Miltenyi), anti-CD123 (Cat. No. 130-115-272, clone REA918, Miltenyi), anti-CD127 (Cat. No. 351363, clone A019D5, Biolegend), anti-CD141 (Cat. No. 344113, clone M80, Biolegend), anti-CD16 (Cat. No. 130-113-395, clone REA423, Miltenyi), anti-CD163 (Cat. No. 333631, clone GHI/61, Biolegend), anti-CD19 (Cat. No. 612916, clone SJ25C1, BD), anti-CD1c (Cat. No. 331537, clone L161, Biolegend), anti-CD20 (Cat. No. 612906, clone 2H7, BD), anti-CD206 (Cat. No. 130-126- 623, clone DCN228, Miltenyi), anti-CD25 (Cat. No. 356145, clone M-A251, Biolegend), anti-CD3 (Cat. No. 130-113-142, clone REA613, Miltenyi), anti-CD38 (Cat. No. 303549, clone HIT2, Biolegend), antiCD39 (Cat. No. 749967, clone TU66, BD), anti-CD4 (Cat. No. 344669, clone SK3, Biolegend), antiCD45 (Cat. No. 130-113-120, clone 5B1, Miltenyi), CD45RA (Cat. No. 740298, clone HI100, BD), antiCD56 (Cat. No. 362513, clone 5.1H11, Biolegend), anti-CD66b (Cat. No. 305111, clone G10F5, Biolegend), CD79b (Cat. No. 612814, clone CB3-1, BD), anti-CD8 (Cat. No. 344759, clone SK1, Biolegend), anti-CXCR3 (Cat. No. 353755, clone G025H7, Biolegend), anti-CXCR5 (Cat. No. 356941, clone J252D4, Biolegend), anti-FOLR2 (Cat. No. 391705, clone 94b/FOLR2, Biolegend), anti-HLA-DR (Cat. No. 307683, clone L243, Biolegend), anti-PD-1 (Cat. No. 329927, clone EH12.2H7, Biolegend), anti-TCF1 (Cat. No. 905115, Cell Signaling), anti-TIM-3 (Cat. No. 345027, clone F38-2E2, Biolegend), anti-TREM2 (Cat. No. FAB17291G, clone 237920, RD), Viability (Cat. No. 423105, Biolegend). .. After obtaining single cell suspensions, bone marrow mononuclear cells were stained with a mix of antibodies for 30 minutes at 4°C, comprising: CD223 Antibody, anti-human, REAfinityTM (Cat. No. 130- 118-549, Miltenyi), BUV395 Mouse Anti-Human CD41a (Cat. No. 740295, BD), BUV496 Mouse AntiHuman CD90 (Cat. No. 741160, BD), BUV661 Mouse Anti-Human CD39 (Cat. No. 749967, BD), BUV737 Mouse Anti-Human CD7 (Cat. No. 753774, BD), Brilliant Violet 421TM anti-human CD34 Antibody (Cat. No. 343610, Biolegend), Brilliant Violet 570TM anti-human CD3 Antibody (Cat. No. 300436, Biolegend), BV605 Mouse Anti-Human CD56 (Cat. No. 562779, BD), Brilliant Violet 650TM anti-human CD16 Antibody (302042, Biolegend), BV786 Mouse Anti-Human CD33 (Cat. No. 740974, BD), PerCP anti-human CD45 Antibody (Cat. No. 368506, Biolegend), PE/FireTM 640 anti-human CD19 Antibody (Biolegend), CD19 CAR FMC63 Idiotype Antibody, REAfinityTM (Cat. No. 130-127-342, Miltenyi), BUV615 Rat Anti-Human CD115 (CSF-1R) (Cat. No. 751279, BD), BB515 Armenian Hamster Anti-ICOS (CD278) (Cat. No. 565880, BD), BUV805 Mouse Anti-Human CD38 (Cat. No.742074, BD), BV510 Mouse Anti-Human CD279 (PD-1) (Cat. No. 563076, BD). .. FCS files were exported using FlowJO software.

    Clinical Proteomics:

    Article Title: Gut Microbiota Modulation through Akkermansia spp . Supplementation Increases CAR T-cell Potency
    Article Snippet: Briefly, after obtaining single-cell suspensions, tumors were stained for 30 minutes at 4°C with a mix of antibodies containing anti-CAR T-cell CD19 (Cat. No. 130-127-342, clone REA1297, Miltenyi), anti-CCR6 (Cat. No. 566130, clone 11A9, BD), anti-CCR7 (Cat. No. 566437, clone 3D12 RUO, BD), anti-CD11b (Cat. No. 301325, clone ICRF44), anti-CD11c (Cat. No. 130-128-247, clone REA618, Miltenyi), anti-CD123 (Cat. No. 130-115-272, clone REA918, Miltenyi), anti-CD127 (Cat. No. 351363, clone A019D5, BioLegend), anti-CD141 (Cat. No. 344113, clone M80, BioLegend), anti-CD16 (Cat. No. 130-113-395, clone REA423, Miltenyi), anti-CD163 (Cat. No. 333631, clone GHI/61, BioLegend), anti-CD19 (Cat. No. 612916, clone SJ25C1, BD), anti-CD1c (Cat. No. 331537, clone L161, BioLegend), anti-CD20 (Cat. No. 612906, clone 2H7, BD), anti-CD206 (Cat. No. 130-126-623, clone DCN228, Miltenyi), anti-CD25 (Cat. No. 356145, clone M-A251, BioLegend), anti-CD3 (Cat. No. 130-113-142, clone REA613, Miltenyi), anti-CD38 (Cat. No. 303549, clone HIT2, BioLegend), anti-CD39 (Cat. No. 749967, clone TU66, BD), anti-CD4 (Cat. No. 344669, clone SK3, BioLegend), anti-CD45 (Cat. No. 130-113-120, clone 5B1, Miltenyi), CD45RA (Cat. No. 740298, clone HI100, BD), anti-CD56 (Cat. No. 362513, clone 5.1H11, BioLegend), anti-CD66b (Cat. No. 305111, clone G10F5, BioLegend), CD79b (Cat. No. 612814, clone CB3-1, BD), anti-CD8 (Cat. No. 344759, clone SK1, BioLegend), anti-CXCR3 (Cat. No. 353755, clone G025H7, BioLegend), anti-CXCR5 (Cat. No. 356941, clone J252D4, BioLegend), anti-FOLR2 (Cat. No. 391705, clone 94b/FOLR2, BioLegend), anti–HLA-DR (Cat. No. 307683, clone L243, BioLegend), anti–PD-1 (Cat. No. 329927, clone EH12.2H7, BioLegend), anti-TCF1 (Cat. No. 905115, Cell Signaling Technology), anti–TIM-3 (Cat. No. 345027, clone F38-2E2, BioLegend), anti-TREM2 (Cat. No. FAB17291G, clone 237920, RD), and Viability (Cat. No. 423105, BioLegend). .. After obtaining single-cell suspensions, BM mononuclear cells were stained with a mix of antibodies for 30 minutes at 4°C, comprising CD223 antibody, anti-human, REAfinity (Cat. No. 130-118-549, Miltenyi); BUV395 Mouse Anti-Human CD41a (Cat. No. 740295, BD); BUV496 Mouse Anti-Human CD90 (Cat. No. 741160, BD); BUV661 Mouse Anti-Human CD39 (Cat. No. 749967, BD); BUV737 Mouse Anti-Human CD7 (Cat. No. 753774, BD); Brilliant Violet 421 anti-human CD34 Antibody (Cat. No. 343610, BioLegend); Brilliant Violet 570 anti-human CD3 Antibody (Cat. No. 300436, BioLegend); BV605 Mouse Anti-Human CD56 (Cat. No. 562779, BD); Brilliant Violet 650 anti-human CD16 Antibody (Cat. No. 302042, BioLegend); BV786 Mouse Anti-Human CD33 (Cat. No. 740974, BD); PerCP anti-human CD45 Antibody (Cat. No. 368506, BioLegend); PE/Fire 640 anti-human CD19 Antibody (BioLegend); CD19 CAR FMC63 Idiotype Antibody, REAfinity (Cat. No. 130-127-342, Miltenyi); BUV615 Rat Anti-Human CD115 (CSF-1R; Cat. No. 751279, BD); BB515 Armenian Hamster Anti-ICOS (CD278; Cat. No. 565880, BD); BUV805 Mouse Anti-Human CD38 (Cat. No. 742074, BD); and BV510 Mouse Anti-Human CD279 (PD-1; Cat. No. 563076, BD). .. FCS files were exported using FlowJo software.

    Article Title: Gut microbiota modulation through Akkermansia spp. supplementation increases CAR-T cell potency
    Article Snippet: Briefly, after obtaining single cell suspensions, tumors were stained for 30 minutes at 4°C with a mix of antibodies containing: anti-CAR-T CD19 (Cat. No. 130-127-342, clone REA1297, Miltenyi), anti-CCR6 (Cat. No. 566130, clone 11A9, BD), anti-CCR7 (Cat No. 566437, clone 3D12 RUO, BD), anti-CD11b (Cat. No. 301325, clone ICRF44), anti-CD11c (Cat. No. 130-128-247, clone REA618, Miltenyi), anti-CD123 (Cat. No. 130-115-272, clone REA918, Miltenyi), anti-CD127 (Cat. No. 351363, clone A019D5, Biolegend), anti-CD141 (Cat. No. 344113, clone M80, Biolegend), anti-CD16 (Cat. No. 130-113-395, clone REA423, Miltenyi), anti-CD163 (Cat. No. 333631, clone GHI/61, Biolegend), anti-CD19 (Cat. No. 612916, clone SJ25C1, BD), anti-CD1c (Cat. No. 331537, clone L161, Biolegend), anti-CD20 (Cat. No. 612906, clone 2H7, BD), anti-CD206 (Cat. No. 130-126- 623, clone DCN228, Miltenyi), anti-CD25 (Cat. No. 356145, clone M-A251, Biolegend), anti-CD3 (Cat. No. 130-113-142, clone REA613, Miltenyi), anti-CD38 (Cat. No. 303549, clone HIT2, Biolegend), antiCD39 (Cat. No. 749967, clone TU66, BD), anti-CD4 (Cat. No. 344669, clone SK3, Biolegend), antiCD45 (Cat. No. 130-113-120, clone 5B1, Miltenyi), CD45RA (Cat. No. 740298, clone HI100, BD), antiCD56 (Cat. No. 362513, clone 5.1H11, Biolegend), anti-CD66b (Cat. No. 305111, clone G10F5, Biolegend), CD79b (Cat. No. 612814, clone CB3-1, BD), anti-CD8 (Cat. No. 344759, clone SK1, Biolegend), anti-CXCR3 (Cat. No. 353755, clone G025H7, Biolegend), anti-CXCR5 (Cat. No. 356941, clone J252D4, Biolegend), anti-FOLR2 (Cat. No. 391705, clone 94b/FOLR2, Biolegend), anti-HLA-DR (Cat. No. 307683, clone L243, Biolegend), anti-PD-1 (Cat. No. 329927, clone EH12.2H7, Biolegend), anti-TCF1 (Cat. No. 905115, Cell Signaling), anti-TIM-3 (Cat. No. 345027, clone F38-2E2, Biolegend), anti-TREM2 (Cat. No. FAB17291G, clone 237920, RD), Viability (Cat. No. 423105, Biolegend). .. After obtaining single cell suspensions, bone marrow mononuclear cells were stained with a mix of antibodies for 30 minutes at 4°C, comprising: CD223 Antibody, anti-human, REAfinityTM (Cat. No. 130- 118-549, Miltenyi), BUV395 Mouse Anti-Human CD41a (Cat. No. 740295, BD), BUV496 Mouse AntiHuman CD90 (Cat. No. 741160, BD), BUV661 Mouse Anti-Human CD39 (Cat. No. 749967, BD), BUV737 Mouse Anti-Human CD7 (Cat. No. 753774, BD), Brilliant Violet 421TM anti-human CD34 Antibody (Cat. No. 343610, Biolegend), Brilliant Violet 570TM anti-human CD3 Antibody (Cat. No. 300436, Biolegend), BV605 Mouse Anti-Human CD56 (Cat. No. 562779, BD), Brilliant Violet 650TM anti-human CD16 Antibody (302042, Biolegend), BV786 Mouse Anti-Human CD33 (Cat. No. 740974, BD), PerCP anti-human CD45 Antibody (Cat. No. 368506, Biolegend), PE/FireTM 640 anti-human CD19 Antibody (Biolegend), CD19 CAR FMC63 Idiotype Antibody, REAfinityTM (Cat. No. 130-127-342, Miltenyi), BUV615 Rat Anti-Human CD115 (CSF-1R) (Cat. No. 751279, BD), BB515 Armenian Hamster Anti-ICOS (CD278) (Cat. No. 565880, BD), BUV805 Mouse Anti-Human CD38 (Cat. No.742074, BD), BV510 Mouse Anti-Human CD279 (PD-1) (Cat. No. 563076, BD). .. FCS files were exported using FlowJO software.



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